483nm CWL, 25mm Dia, 31nm Bandwidth, OD 6 Fluorescence Filter

Product Family Description

• Common Wavelengths for Popular Fluorophores
• Excitation Filters and Emission Filters Available
• >93% Transmission
• >OD 6 Blocking, <3% from Edge of Bandwidth
• Fluorescence Filter Sets and Pre-Mounted Fluorescence Filter Cube Sets Also Available

Our TECHSPEC® Fluorescence Bandpass Filters are perfectly matched to common fluorophores used in fluorescence imaging applications. Excitation Filters and Emission Filters are available featuring >93% transmission and >OD 6 blocking outside of the passband. These filters are also ideal components in spectroscopy and clinical chemistry applications, as well as biotech instruments such as DNA sequencers and polymerase chain reaction (PCR) testing platforms. Each filter is hard sputtered coated and mounted in a black anodized ring.

For a complete list of compatible fluorophores for each Excitation or Emission Filter, view Fluorophores and Optical Filters for Fluorescence Microscopy.

Excitation filters and emission filters are crucial components in microscopy and imaging applications. Engineered with cutting-edge technology, these filters are designed to enhance signal-to-noise ratios, improve contrast, and deliver unparalleled performance in optical imaging applications. Excitation filters isolate the specific wavelength of light that excites the fluorophores in the sample, enabling them to emit fluorescence. On the other hand, emission filters allow only the emitted fluorescence to pass through while blocking the excitation light, producing a clear and accurate image. These filters are vital in ensuring precise imaging and accurate fluorescence detection in various microscopy techniques. 

These fluorescence bandpass filters offer a range of exceptional features that make them ideal for various fluorescence microscopy systems and imaging techniques. Each filter is manufactured with high-quality materials and state-of-the-art optical coatings, ensuring exceptional spectral performance and minimal light leakage. This results in superior image clarity and accuracy.

Bandpass filters are optimized for maximum transmission within a specific wavelength range, effectively isolating fluorescence signals from background noise, enhancing sensitivity and enabling more accurate detection of fluorophores. The excitation filter design ensures that the filters provide excellent transmission of the desired excitation wavelength, achieving over 93% (typical) transmission efficiency while maintaining a sharp spectral cutoff to minimize the transmission of other wavelengths to less than 0.001%. The emission filters match the performance of excitation filters, with over 93% (typical) transmission of the desired emission wavelength and an equally sharp spectral cutoff, ensuring minimal interference from other wavelengths. Achieve optimal signal intensity with minimal loss of fluorescence signal with these filters' high transmission efficiency. 

These filters are designed to be widely compatible with microscopes from Nikon, Olympus, and Zeiss, making them suitable for a range of imaging applications, including confocal microscopy, widefield fluorescence microscopy, and multiphoton microscopy. Whether you're conducting cellular imaging, live cell imaging, or tissue imaging, our filters are engineered to deliver consistent and reliable results. 

Fluorescence microscopy, immunofluorescence, FRET, FISH, live cell imaging, and high-content screening are common applications of fluorescence imaging. These techniques use fluorescent molecules or dyes to visualize a sample's specific structures, molecules, or DNA sequences. Live cell imaging observes dynamic processes in living cells, while high-content screening screens large numbers of samples for specific features or characteristics.

Whether studying cellular dynamics, protein localization, or molecular interactions, these filters empower sharper, more accurate results confidently. Their robust high-performance design facilitates accurate and efficient fluorescence imaging and contribute to the reliability and repeatability of scientific experiments. Built to withstand the rigors of laboratory use, this ensures long-lasting performance and reliability for research and imaging applications.

 Explore our range of filters today and unleash the full potential of your fluorescence microscopy setup.